Scientist I/II, In Vivo Pharmacology

Primary Responsibilities • Design and execute in vivo studies that de-risk novel targets, using genetic knockdown/overexpression and tool molecules to test target–phenotype links

• Outsource or internally establish rodent cardiac disease models, including transgenic lines and surgical models such as TAC and MI

• Run therapeutic screening studies that rank candidates on efficacy, dose-response, durability, and identify hit molecules for advancement

• Dose by multiple routes (IV, IP, SC, IM, PO, retro-orbital) and by osmotic minipump across longitudinal studies

• Lead study takedowns: necropsy, blood and tissue collection, and processing for histology, molecular, and ex vivo endpoints, including Langendorff cardiac dissociation

• Partner with Translational Sciences to establish bioanalytical methods for biodistribution and target engagement in rodent tissues, and align on models with clinical endpoints/biomarkers

• Analyze, interpret, and present data to project teams; keep study records in the ELN in line with IACUC and animal welfare standards

• Manage study logistics, manage budgets, flag capacity or timeline risks early, and ideate novel methods for pharmacology and physiology

Required Skills and Qualifications • PhD in physiology, pharmacology, cardiovascular biology, or a related field with 0–3 years of postdoctoral or industry experience; or MS with 5+ years of industry experience

• Hands-on rodent experience: handling, multi-route dosing, blood collection, survival surgery, and necropsy

• Track record using rodent disease models for target validation or efficacy testing of molecules

• Proven understanding of power analyses, methods of randomizing animal models and appropriate uses of statistical methods

• Strong data analysis skills (e.g., GraphPad) and clear written and verbal communication

• Organized and independent; able to run several longitudinal studies at once within a collaborative team

• Ability to regularly work onsite at HQ in New York City

Preferred Skills and Qualifications • Industry experience in a small, fast-paced biotech is highly preferred

• Working knowledge of qPCR/ddPCR for vector/mRNA, ELISA/LC-MS for ASO, RNAscope/IHC for tissue localization, WB/ ELISA/MSD for protein detection

• Hands-on echocardiography in mice and rats (e.g., Vevo)

• ECG and blood pressure telemetry implantation and analysis in mice and rats (e.g., Ponemah)

• Cardiac surgical models (TAC, MI) and invasive hemodynamics (PV-loop)

• Demonstrated experience with models of neurodegenerative disease, particularly ataxia or cerebellar disorders, including execution and interpretation of rotarod, gait analysis, balance beam, grip strength, and longitudinal behavioral studies

• In vivo experience working with and advancing AAV or oligonucleotide therapeutics

• Experience selecting and evaluating CROs to perform animal studies

• AALAS certification or equivalent